WET Training – Freshwater – Video Series Guidebook

This EPA report accompanies its video training for conducting Ceriodaphnia dubia (freshwater water flea) survival and reproduction toxicity tests (EPA, 2006). The test method is found in Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, Fourth Edition (EPA, 2002). The test is adapted from methods developed by Dr. Donald Mount and Teresa Norberg-King of EPA’s Mid-Continent Ecology Division (MED), in Duluth, Minnesota. The material presented in both the video and this report summarizes the methods but does not replace a thorough review and understanding of the methods by laboratory personnel before conducting the test.